Involvement of Flagella-Driven Motility and Pili in Pseudomonas aeruginosa Colonization at the Air-Liquid Interface
نویسندگان
چکیده
Many aerobic microorganisms can colonize at the air-liquid interface and form a multicellular structure, known as a pellicle. In this study, the involvement of motility and attachment traits in the Pseudomonas aeruginosa pellicle formation process was investigated. Flagella- and flagellar-motor-deficient mutants exhibited delayed pellicle formation and unusual pellicle morphology, indicating the large contribution of flagella-driven motility to structural development of the pellicle. A pili-deficient mutant showed normal pellicle formation properties, while the disruption of the pilus gene in the flagella-deficient mutant restored normal pellicle morphology. These results indicate that flagella and pili play key roles in P. aeruginosa pellicle development.
منابع مشابه
Pseudomonas aeruginosa exhibits sliding motility in the absence of type IV pili and flagella.
Pseudomonas aeruginosa exhibits swarming motility on 0.5 to 1% agar plates in the presence of specific carbon and nitrogen sources. We have found that PAO1 double mutants expressing neither flagella nor type IV pili (fliC pilA) display sliding motility under the same conditions. Sliding motility was inhibited when type IV pilus expression was restored; like swarming motility, it also decreased ...
متن کاملThe recognizing of fli C gene in Pseudomonas aeruginosa isolated from clinical sample with PCR
Abstract Background and objectives: Pseudomonas aeruginosa as an opportunistic pathogen can establish lethal infections in immunocompromised patients or those exposed to predisposing factors. This bacterium contains a single polar flagellum causing motility, chemotaxis and colonization in acute phase of infection. The flagella filament is made up of a structural protein called flagellin. This s...
متن کاملCross-regulation of Pseudomonas motility systems: the intimate relationship between flagella, pili and virulence.
Pseudomonas aeruginosa navigates using two distinct forms of motility, swimming and twitching. A polar flagellum and Type 4 pili power these movements, respectively, allowing P. aeruginosa to attach to and colonize surfaces. Single cell imaging and particle tracking algorithms have revealed a wide range of bacterial surface behaviors which are regulated by second messengers cyclic-di-GMP and cA...
متن کاملOrganelles as virulence factors of an opportunistic pathogen Pseudomonas aeruginosa
Type IV pili (T4P) and flagellum are well known virulence factors of an opportunistic pathogen Pseudomonas aeruginosa. T4P pili provide twitching motility, adherence to biotic/abiotic surfaces, intercellular association that is essential to biophilm formation, and are implicated in chemotaxis, bacteriophage adsorption and DNA uptake. Flagellum provides swimming/swarming motilities and chemotaxi...
متن کاملNanoscale Adhesion Forces of Pseudomonas aeruginosa Type IV Pili
A variety of bacterial pathogens use nanoscale protein fibers called type IV pili to mediate cell adhesion, a primary step leading to infection. Currently, how these nanofibers respond to mechanical stimuli and how this response is used to control adhesion is poorly understood. Here, we use atomic force microscopy techniques to quantify the forces guiding the adhesion of Pseudomonas aeruginosa ...
متن کامل